Precision Support, Perfect Sectioning —— Your Reliable Frozen Sectioning Solution
Composition and Use
Key Composition
A water-soluble blend primarily composed of polyethylene glycol (PEG) and polyvinyl alcohol (PVA). Select formulations are further supplemented with polyols such as glycerol and propylene glycol.
Core Purpose
Specially engineered to provide structural tissue support and section protection throughout the cryosectioning workflow.
Tissue Support Performance
Boosts tissue continuity during frozen sectioning, effectively reducing wrinkling, folding and sample fragmentation.
Water-Soluble Formulation
Fully dissolves in water at the section flotation stage, introducing no background interference to downstream staining procedures.
Application Scenarios
Widely used in immunohistochemistry (IHC), immunofluorescence (IF), rapid clinical pathological staining and diagnosis, in situ hybridization (ISH) and a broad range of molecular pathology assays.
Procedure
Tissue Soaking: Remove tissue blocks from sucrose solution, place in a 1:1 mixture of 20 g/L sucrose and OCT embedding medium, and soak at room temperature for 2 hours.
Embedding: Transfer to pure OCT embedding medium and soak at room temperature for 4–6 hours; fresh embedding medium can be replaced if necessary.
Section Storage: Section with a cryostat (typically at -22°C) at a thickness of approximately 10 μm. Mount sections on glass slides and store at -20°C in a refrigerator.
Perfect Section Integrity
Our polymer formulation cures at a rate matched to tissue hardness, significantly reducing wrinkling, folding and fragmentation to deliver consistent, high-quality sections.
Crystal-Clear Staining Quality
This formulation leaves zero residue on glass slides, eliminating background staining interference and delivering clear, distinct results.
Fast and Efficient Workflow
Fast curing shortens embedding wait time, accelerates sample preparation and boosts laboratory productivity.
Designed for Convenience and Safety
Features a precision-engineered bottle opening for controlled dispensing and secure storage.
Proven and Versatile
Reliable performance across diverse samples: fresh/fixed tissues and cell pellets. Stable at room temperature with a 24-month shelf life and reliable supply.